CRL-2572 A3 人T淋巴細(xì)胞白血病細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件!

CRL-2572 A3 人T淋巴細(xì)胞白血病細(xì)胞 的詳細(xì)介紹

ATCC® Number:	CRL-2572?	Price:	$338.00
Designations: I 2.1 Depositors: J Blenis Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: suspension Organism: Homo sapiens (human) Morphology: lymphoblast Source: Disease: acute T cell leukemia Cell Type: T lymphocyte; Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. DNA Profile (STR): Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,11 D16S539: 11 D5S818: 9 D7S820: 8,10 THO1: 6,9.3 TPOX: 8,10 vWA: 17,18 Gender: male Comments: The I 2.1 cell line is a FADD mutant of the wild-type Jurkat cell line A3 (see ATCC -CRL-2570). Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to theframeshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody. [51527] ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks. [51529] Two of these ICR-191 treated clones have been deposited at the ATCC . They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD. Unlike the parental line A3 that does express FADD, the I 2.1 cell line does not express FADD protein by immunoblot analysis and is completely resistant to Fas-induced death. [51529] Complementation of the I 2.1 cell lines with wild-type FADD restores Fas-mediated apoptosis. Fas activation of caspase-2, caspase-3, caspase-7, and caspase-8 is completely defective in the FADD mutant cell line I 2.1. [51529] This cell line can be used to study the role of FADD in apoptotic signaling pathways in the absence of overxpression. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cell density between 2 X 10(5) and 2 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-2570 References: 51527: Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801 51529: Juo P, et al. FADD is required for multiple signaling events downstream of the receptor Fas. Cell Growth Differ. 10: 797-804, 1999. PubMed: 10616904 51530: Juo P, et al. Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases. Mol. Cell. Biol. 17: 24-35, 1997. PubMed: 8972182

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人胰腺癌細(xì)胞 HPAC CRL-2119
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CRL-6323 B16F1 小鼠黑色素瘤細(xì)胞
HTB-58 SK-MES-1 人肺鱗癌細(xì)胞
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HTB-79 Capan-2 人胰腺癌細(xì)胞
BALL-1 人B淋巴細(xì)胞急性白血病細(xì)胞
CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
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CRL-2539 4T1 小鼠乳腺癌細(xì)胞
CRL-1442 BRL-3A 大鼠肝細(xì)胞
BRL 大鼠肝細(xì)胞
RSC96大鼠雪旺細(xì)胞
Isk 人子宮內(nèi)膜癌細(xì)胞
HTB-113 hec-1-b 人子宮內(nèi)膜腺癌細(xì)胞
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Pt-K2 袋鼠腎細(xì)胞
BS-C-1 非洲綠猴腎細(xì)胞
A498人腎癌細(xì)胞
3AO人卵巢癌細(xì)胞
iec-6 大鼠小腸隱窩上皮細(xì)胞
u87MG 人腦星形膠質(zhì)母細(xì)胞瘤
Renca 小鼠腎癌細(xì)胞
nci-h929 骨髓瘤細(xì)胞
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opm-2 骨髓瘤細(xì)胞
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MSTO-211H 人肺癌細(xì)胞
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LS 180人結(jié)腸腺癌細(xì)胞
HuTu-80十一指腸腺癌
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NCI-H520 人肺鱗癌細(xì)胞
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RK-13 兔腎細(xì)胞系