特別提示:包括兔抗Ubiquitin抗體在內(nèi),本公司的所有產(chǎn)品僅可用于科研實驗,嚴禁用于臨床醫(yī)療及其他非科研用途!
產(chǎn)品名稱:兔抗Ubiquitin抗體
英文名稱:Ubiquitin Rabbit Polyclo
nal Antibody
產(chǎn)品貨號:YT766
產(chǎn)品規(guī)格:50μl
本品為來源于兔的抗Ubiquitin的多克隆抗體。
宿主:兔
克隆性:多克隆抗體
用途:WB,ICC, IHC,IF
交叉反應(yīng)性:人,小鼠,大鼠
分子量:10KDa
推薦稀釋比例:WB(1:500-1:1000),IF(1:200),IHC(1:200),ICC(1:200)
about this Antibody
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Name
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Ubiquitin Rabbit Polyclonal Antibody
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Category
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Polyclonal antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide affinity purified
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about the Immunogen
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Immunogen
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This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to Ubiquitin.
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Gene ID
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7314
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SwissProt
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P62988
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Synonyms
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40S ribosomal protein S27a; CEP80; Ribosomal protein S27a; RPS27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP80; UBIQ; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80; ubiquitin-CEP80
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Category
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Ubiquitin and Ubiquitin-like proteins
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Background
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Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B.
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注意事項:
1. 如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(xué)(ICC)等實驗,請注意回收使用過的稀釋抗體?;厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體,包括已經(jīng)使用過的稀釋抗體,請4℃保存。
2. 回收后重復(fù)使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況,則可以考慮廢棄該抗體。
3. 提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(xué)(ICC)等適當用途。如果希望獲得zuì佳的檢測效果,請考慮使用上述檢測專用的一抗稀釋液。
儲存條件:-20℃,有效期一年。
根據(jù)您的關(guān)注的
兔抗Ubiquitin抗體,您可能還對以下產(chǎn)品有需求:
名稱:肌酸激酶M型抗體
貨號:K12197
規(guī)格:0.1ml|0.2ml
宿主物種:兔
交叉反應(yīng):人,小鼠,大鼠,狗,豬,馬,兔,斑馬魚
克隆類型:多克隆
分 子 量:43kDa
濃 度:1mg/1ml
亞 型:IgG
細胞定位:細胞漿
免 疫 原:KLH co
njugated synthetic peptide derived from human Creatine
純化方法:蛋白A親和純化
產(chǎn)品應(yīng)用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
尚未測試其他應(yīng)用;
實際稀釋濃度可根據(jù)用戶的具體用途決定。
儲 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件:4℃運輸,-20℃保存,避免反復(fù)凍融
應(yīng)用領(lǐng)域:心血管 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 糖尿病
名稱:乳腺癌相關(guān)抗原抗體
貨號:K12494
規(guī)格:0.1ml|0.2ml
宿主物種:兔
交叉反應(yīng):人
克隆類型:多克隆
分 子 量:138kDa
濃 度:1mg/1ml
亞 型:IgG
細胞定位:細胞核 細胞漿 細胞膜 分泌型蛋白
免 疫 原:KLH co
njugated synthetic peptide derived from human CA153/EM
純化方法:蛋白A親和純化
產(chǎn)品應(yīng)用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200 (石蠟切片需做抗原修復(fù))
尚未測試其他應(yīng)用;
實際稀釋濃度可根據(jù)用戶的具體用途決定。
儲 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件:4℃運輸,-20℃保存,避免反復(fù)凍融
應(yīng)用領(lǐng)域:腫瘤 細胞生物 t-淋巴細胞 上皮細胞
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關(guān)注
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